MYOGH

Prompt

The protein features an AAA C-terminal domain involved in DNA recombination.

AI Generated Sequence

292 AAs | 32.37 kDa | 34.19 kDa (with tag)

MSDLFGADDAPLADRLRPRTLDEVVGQDHLLGEDGPLRRMLESGRLSSMIFWGPPGTGKTTLARLLAGASDLHFEQISAVFSGVADLKKVFEAARQRRAMGQRTLLFVDEIHRFNRAQQDAFLPHVEDGTVTLVGATTENPSFELNSALLSRAQVYVLNALDEAALTRLLERAEETERGLGKLPLDEEARALLIAMADGDGRFLLNLLEQLLAARPEEVFDTAGLAERVQRRAPRFDKSQDEHYNLISALHKSVRGSDPDAALYRLARMLDAGEDPRYLARRLVRMASEDIG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

69.26

AAComp

89.94

DiffDock Confidence

-0.61

Seqdif (SeqID)

74.09 (MCO5084112)

Structdif (F-Target)

65.92 (2chg C)

ProtNLM

Name	Score
Replication-associated recombination protein A	0.566
AAA family ATPase	0.288
Replication-associated recombination protein RarA	0.051
AAA domain-containing protein	0.027
Recombination factor protein RarA	0.024
ATPase AAA	0.007
ATPase	0.006
Recombination protein MgsA	0.003
Recombinase RarA	0.003
ATPase, AAA family	0.003

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.06 (Lunatic)	3	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.02 (Lunatic)	1	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.18 (Lunatic)	9	79.1	N/A	N/A
Tierra	T4	Biologics Optimized System	0.24 (Lunatic)	12	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

43_-1.04t | 80