MPBDZ

Prompt

The protein features a ATP-dependent RNA helicase DEAD-box, conserved site and a cellular macromolecule catabolic process.

AI Generated Sequence

292 AAs | 31.72 kDa | 33.54 kDa (with tag)

MTTTPFADLPLSPALLAALDALGFEEPTPIQAQALPALLAGRDVIGQAKTGSGKTAAFGLPLLQRIDPARHAPQALVLCPTRELAIQVAREIRRLARDIPNIKVLTLYGGQPIGPQIRSLEHGAHIVVGTPGRILDHLERGTLKLDGLNTLVLDEADRMLDMGFQDAIDAILAQTPKDRQTLLFSATFPPAIAKIARRQMRDPVEITVAAEHADAPIEQRFWEVAPGERLHALGRLLDHFEPASALAFCNTKRDVDTLAATLQARGISALALHGELDQRERDQVLMQFANRS

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

77.76

AAComp

89.95

DiffDock Confidence

-1.12

Seqdif (SeqID)

67.01 (WP_115537694)

Structdif (F-Target)

67.2 (5gju A)

ProtNLM

Name	Score
ATP-dependent RNA helicase DbpA	0.426
DEAD/DEAH box helicase	0.379
3.6.4.13	0.062
ATP-dependent RNA helicase	0.049
DEAD/DEAH box helicase domain protein	0.011
ATP-independent RNA helicase DbpA	0.007
RNA helicase	0.007
DEAD/DEAH box helicase domain-containing protein	0.004
Cold-shock DEAD box protein A	0.004
DEAD-box ATP-dependent RNA helicase CshA	0.004

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	1.16 (Lunatic)	58	48.6	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.13 (Lunatic)	56.5	79.8	N/A	N/A
Tierra	T3	Biologics Optimized System	0.33 (Lunatic)	16.5	28.8	N/A	N/A
Tierra	T4	Biologics Optimized System	0.52 (Lunatic)	26	93.8	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	55.2	55.9

Data

58_-1.18t | 70