MDRU7

Prompt

The protein features a metallo-sulfur cluster assembly and a Mrp, conserved site.

AI Generated Sequence

297 AAs | 31.28 kDa | 33.1 kDa (with tag)

MSEEQVRAALETVIDPELKKDIVSLGFVKDVEVADGKVSFTVELGEKGDPTLEPIRAEAEKAVRELGVGEKAEVTLTAEIKPGHAQEGKVSLPGVKNIIAVASGKGGVGKSTVAVNLALALAQEGKKVGILDADINGPSIPRMMGIEGKPEVSDGNTIIPLEAHGVKSMSMGFLVEKDEPMIWRGPMVSSALTQFLRETDWGELDLLVVDMPPGTGDVQLTLAQSVPLAGAVIVGTPQEIALIDARKGIAMFKKVGVPIVGVVENMSHFICPNCGHEEEIFGKGGAKETAEEHGIEF

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

72.12

AAComp

89.9

DiffDock Confidence

-1.16

Seqdif (SeqID)

59.67 (HEV8026153)

Structdif (F-Target)

65.42 (2ph1 A)

ProtNLM

Name	Score
Iron-sulfur cluster carrier protein	0.774
Iron-sulfur cluster carrier protein ApbC	0.201
FeS_assembly_P domain-containing protein	0.004
Mrp/NBP35 family ATP-binding protein	0.003
[4Fe-4S] cluster assembly scaffold protein Mrp (	0.002
ATP-binding protein	0.002
DUF59 domain-containing protein	0.002
Chromosome partitioning protein	0.002
MRP family ATP-binding protein	0.002
P-loop NTPase	0.001

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.02 (Lunatic)	1	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.09 (Lunatic)	4.5	200	N/A	N/A
Tierra	T3	Biologics Optimized System	0.13 (Lunatic)	6.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.01 (Lunatic)	0.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

66_-1.28 | 77