MS1ZT

Prompt

The protein features metallo-sulfur cluster assembly and resembles the iron-sulfur protein NUBPL.

AI Generated Sequence

297 AAs | 31.31 kDa | 33.13 kDa (with tag)

MSEEQVRAALETVIDPELKKDIVSLGFVKDVEVADGKVSFTVELGEKGCPFLEPIRAEAEKAVRELGVGEKAEVTLTAEIKPGHAQEGKVSLPGVKNIIAVASGKGGVGKSTVAVNLALALAQEGKKVGILDADIYGPSIPRMLGIEGKPEVSDGNTIIPLEAHGVKSMSMGFLVEKDEPMIWRGPMVSSALTQFLRETDWGELDLLVVDMPPGTGDVQLTLAQSVPLAGAVIVGTPQEVALIDARKGIAMFKKVGVPIVGVVENMSHFICPNDGHEEEIFGKGGAKKMAEEAGIEF

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

65.57

AAComp

89.88

DiffDock Confidence

-1.19

Seqdif (SeqID)

60.6 (OIO59206)

Structdif (F-Target)

65.33 (2ph1 A)

ProtNLM

Name	Score
Iron-sulfur cluster carrier protein	0.706
Iron-sulfur cluster carrier protein ApbC	0.228
DUF59 domain-containing protein	0.011
Mrp/NBP35 family ATP-binding protein	0.010
FeS_assembly_P domain-containing protein	0.009
Chromosome partitioning protein	0.008
MRP family ATP-binding protein	0.007
P-loop NTPase	0.003
Chromosome partitioning protein ParA	0.002
ATP-binding protein	0.002

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.25 (Lunatic)	12.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.07 (Lunatic)	3.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.12 (Lunatic)	6	100	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	72.4	72.9

Data

5_-0.74 | 27