MYLZR

Prompt

This protein functions as an RNA helicase and is involved in processes related to symbiotic interactions.

AI Generated Sequence

372 AAs | 40.77 kDa | 42.59 kDa (with tag)

MSFSELGLSEPLLKAVEEMGYTKPTPIQQQAIPAILEGRDVLAIAQTGTGKTAAFTLPILQRLSSGRPRRRGVRALVLAPTRELAAQVGENFRTYGKHLRLRTLAVYGGVSIGPQIRALERGVDILVATPGRLLDHLERGAVKLSGVEILVLDEADRMLDMGFIHDIEKILSLLPKSRQTLLFSATIPPEVRKLADKFLHNPVRIEVARPASTAETIEQKVVKVDKSQKTELLVHLLRDTGREQVLVFTRTKHGADRLAKQLTRDGISAEAIHGDKSQGARLRALADFKSGSVRILVATDVAARGIDIPQLPHVVNYDLPHNAEDYVHRIGRTGRAGAEGLAISLVTADEMRNLRAIERLIGQTLPRKEIPG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

74.3

AAComp

93.4

DiffDock Confidence

-1.89

Seqdif (SeqID)

67.02 (MCI4410543)

Structdif (F-Target)

88.25 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.372
ATP-dependent RNA helicase RhlE	0.211
RNA helicase	0.147
ATP-dependent helicase	0.079
ATP-dependent RNA helicase	0.065
DEAD/DEAH box helicase domain protein	0.024
3.6.4.13	0.022
Superfamily II DNA/RNA helicase	0.019
Superfamily II DNA and RNA helicase	0.013
DNA/RNA helicase, superfamily II	0.007

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.45 (Lunatic)	22.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.3 (Lunatic)	15	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.01 (Lunatic)	0.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.44 (Lunatic)	22	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

32_-1.00t | 73