MKV9B

Prompt

The protein features RNA helicase activity and is part of the host cell.

AI Generated Sequence

299 AAs | 33.06 kDa | 34.88 kDa (with tag)

MSFEDLGLSEPLLKAVEELGYTEPTPIQQQAIPAVLEGRDVLAIAQTGTGKTAAFTLPILQRLSKGRARPRGQPRALILAPTRELAAQVSENVKAYSKHLPLRSLAVYGGVSINPQIDALRRGVDILIATPGRLLDHYERGNIHLSQLEILVLDEADRMLDMGFIPDIRKILSLLPKTRQTLLFSATMPPEIRKLADTFLNNPVEISVARPNTAAETIEQSVYPVDKSRKPALLSHLLRTENWEQALVFTRTKHGANRLAKELERDGISAEAIHGDKSQGARLRALEDFKSGRIRVLAE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

76.61

AAComp

93.4

DiffDock Confidence

-1.23

Seqdif (SeqID)

68.69 (MBD3610226)

Structdif (F-Target)

67.31 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.650
RNA helicase	0.125
ATP-dependent RNA helicase RhlE	0.095
DEAD/DEAH box helicase domain protein	0.023
ATP-dependent helicase	0.019
ATP-dependent RNA helicase	0.017
3.6.4.13	0.012
Superfamily II DNA/RNA helicase	0.011
DEAD/DEAH box helicase domain-containing protein	0.008
Superfamily II DNA and RNA helicase	0.007

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	1.1 (Lunatic)	55	42.6	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.51 (Lunatic)	25.5	79.2	N/A	N/A
Tierra	T3	Biologics Optimized System	0.78 (Lunatic)	39	20.6	N/A	N/A
Tierra	T4	Biologics Optimized System	0.58 (Lunatic)	29	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	80.8	low confidence

Data

50atp_-1.48 | 101