MSCMP

Prompt

The protein features a RNA helicase activity and a viral process.

AI Generated Sequence

296 AAs | 32.38 kDa | 34.2 kDa (with tag)

MSFADLGLADPLLKAIEELGYTEPTPIQAQAIPPVLQGRDVLAAAQTGTGKTAAFALPILQRLSKGQPRPRHQVRALVLAPTRELAAQVAENIKDYSKHLNLKSLAVYGGVKIGPQIRALERGVDILVATPGRLLDHFEQGKVNLSKLEILVLDEADRMLDMGFIHDIKKILSLLPKTRQTLLFSATMPPEIRKLAQTILHNPVEIEVAPRNTTAETIEQSVYPVDKERKAALLSHLIRGKNPEQALVFSNTKRGADAVAKELERDGISASAIHGDKDQAQRLAALEDFKSGRIRV

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

75.92

AAComp

93.51

DiffDock Confidence

-1.19

Seqdif (SeqID)

66.89 (WP_199467282)

Structdif (F-Target)

67.89 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.707
ATP-dependent RNA helicase RhlE	0.082
RNA helicase	0.063
ATP-dependent RNA helicase	0.045
ATP-dependent helicase	0.029
DEAD/DEAH box helicase domain protein	0.017
Superfamily II DNA/RNA helicase	0.010
DEAD/DEAH box helicase domain-containing protein	0.006
3.6.4.13	0.006
Superfamily II DNA and RNA helicase	0.005

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	5.72 (Lunatic)	286	62.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.27 (Lunatic)	13.5	98.4	N/A	N/A
Tierra	T3	Biologics Optimized System	4.98 (Lunatic)	249	40.8	N/A	N/A
Tierra	T4	Biologics Optimized System	1.5 (Lunatic)	75	95.5	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	75.7	75

Data

42_-1.01 | 46