MUWA8

Prompt

The protein features a DEAD/DEAH box helicase domain and a DNA replication.

AI Generated Sequence

297 AAs | 32.15 kDa | 33.97 kDa (with tag)

MSFADLGLAPELLAAVEAAGYTAPTPIQAQAIPHVLAGRDVLGIAQTGTGKTAAFSLPMLQRLSRGRPARPRAPRALVLAPTRELAAQVAENLRAYGKHLPLRTLALIGGVSFGPQIRALERGVDVLIATPGRLLDHFERGAVLLSGVEVLVLDEADRMLDMGFIPDIRRICALVPRTRQTLFFSATMPPEIRRLADTFLHDPVRIEVARPASAAETVTQRVVPVGHARKREALVRLLREGELEQALVFTRTKHGADRLAKELERDGISAAAIHGDKSQGARTRALADFRAGRVRVL

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

75.43

AAComp

88.99

DiffDock Confidence

-1.34

Seqdif (SeqID)

68.44 (WP_052348177)

Structdif (F-Target)

67.35 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.692
RNA helicase	0.176
ATP-dependent RNA helicase RhlE	0.030
ATP-dependent RNA helicase	0.021
DEAD/DEAH box helicase domain protein	0.018
ATP-dependent helicase	0.015
Superfamily II DNA/RNA helicase	0.011
Superfamily II DNA and RNA helicase	0.006
3.6.4.13	0.005
DEAD/DEAH box helicase domain-containing protein	0.005

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	5.49 (Lunatic)	274.5	39.6	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.72 (Lunatic)	86	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.07 (Lunatic)	3.5	30.6	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

100adp_-1.25 | 43