M929Q

Prompt

Adenylate kinase belongs to its specific subfamily. It is involved in the metabolic process of purine-containing compounds.

AI Generated Sequence

186 AAs | 20.82 kDa | 22.64 kDa (with tag)

MPRFVVVTGIPGVGKTTLVQEALLEREGERFKLVNFGNVMFEEAAKEGYVAHRDEMRKLPLETQRQLQKAAAGRIVAVAREGPVVVDTHASIKTPHGYLPGLPPEVLERLNPDLVVVIEADPQEILGRRLRDETRTRDIETEEQIERHEQMNRAASMAYAVMTGAVIKIVENRDGKEEEAVQELLA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.76

AAComp

88.93

DiffDock Confidence

-1.07

Seqdif (SeqID)

62.9 (WP_013747832)

Structdif (F-Target)

96.84 (3h86 6)

ProtNLM

Name	Score
AK	0.323
Adenylate kinase	0.262
2.7.4.3	0.212
ATP-AMP transphosphorylase	0.196
AAA family ATPase	0.001
Adenylate monophosphate kinase	0.001
Nucleoside monophosphate kinase	0.000
ADK_lid domain-containing protein	0.000
Dual activity adenylate kinase/ATPase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.43 (Lunatic)	21.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.72 (Lunatic)	36	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.19 (Lunatic)	9.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.4 (Lunatic)	20	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

68atp_-1.03t | 29