M7ZLJ

Prompt

This protein from the Green anole has DNA repair and helicase activities, binding and hydrolyzing ATP.

AI Generated Sequence

298 AAs | 33.24 kDa | 35.06 kDa (with tag)

MSLLLLPTRELALQIEEELRRDSRRLGLRHHALLGGTPIAKQIELLREGVQILVATPGRLEDLVRQKVLRLGQVRTLVLDEAHQMLDMDFHPDIRRIGRMLPPDRQTLLFSATLPEEIARLAREFMRNPVEIEVDSATALANLTQHLVLVDESQKKAELLRRLLAEGEGQALVFARTKRRAAELAQELAAAGFRVAALHGGKSQHARERALARFKEGEVQILVATDVAARGIDIPGVSHVINYDLPNVPEAYIHRIGRTARAGREGVAITFVTEEDVALLRAIERAARITIRPLEVEG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.56

AAComp

88.93

DiffDock Confidence

-1.44

Seqdif (SeqID)

51.52 (MCF6272081)

Structdif (F-Target)

90.71 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.500
ATP-dependent RNA helicase RhlE	0.084
DEAD/DEAH box helicase domain protein	0.080
ATP-dependent RNA helicase	0.080
ATP-dependent helicase	0.057
DEAD-box ATP-dependent RNA helicase CshA	0.034
RNA helicase	0.021
3.6.4.13	0.018
DEAD/DEAH box helicase domain-containing protein	0.012
ATP-dependent RNA helicase SrmB	0.010

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	0.228 (A660nm)	29.64	>95	N/A	N/A

Data

17_-1.00t | 19