M4GK5

Prompt

This protein is involved in RNA processing and ATP binding, particularly in RNA helicase activity within ribonucleoprotein complexes.

AI Generated Sequence

298 AAs | 32.02 kDa | 33.84 kDa (with tag)

MSFSDLGLSDPLLRAVTELGFTEPTPIQAQAIPAVLAGRDVLGIAQTGSGKTAAFVLPILQRLDRGRARGGGPQALILAPTRELAAQIAENLATLAKHTRLRTLAVFGGVSIGRQIKALERGVDILVATPGRLLDHLERGALKLDRVETLVLDEADRMLDMGFIHDLTKILKLTPRERQTLLFSATMPKEIRRLAEKFLRDPVEIEVAPAATPAETITQWVVAVDQAEKPRLLTHLLARGEPRMALVFTRTKHGADRVAKLLEAAGIPAAALHGDKSQGARTAALAAFKAGRVRALVA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

75.69

AAComp

89.39

DiffDock Confidence

-1.1

Seqdif (SeqID)

59.2 (NRA72102)

Structdif (F-Target)

67.15 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.788
ATP-dependent RNA helicase	0.062
RNA helicase	0.052
ATP-dependent RNA helicase RhlE	0.027
ATP-dependent helicase	0.015
DEAD/DEAH box helicase domain protein	0.014
Superfamily II DNA/RNA helicase	0.010
3.6.4.13	0.006
Superfamily II DNA and RNA helicase	0.003
Helicase	0.003

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.99 (Lunatic)	49.5	72.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	3.71 (Lunatic)	185.5	70	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

54atp_-1.37 | 22