MR2ZR

Prompt

The protein features a Adenylate kinase subfamily and a purine ribonucleotide metabolic process.

AI Generated Sequence

214 AAs | 22.93 kDa | 24.75 kDa (with tag)

MNIVLLGPPGAGKGTQARRLVEKFGIVQISTGDMLRAAVAAGTEVGKKAAEVMAAGELVPDEITNAIVEERIAQPDKAKGFIFDGFPRTVAQAEATKAMLADKGLKLDAVIELNVDEDALVERIAGRITHANEGAGIHIEFKPPKVEGVVDKTGGTEFKRRDDDNEETVKTRLEVYREQTAPLIDFYRAKGLLVNIDGMADIDAVTADIDKVLG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.59

AAComp

89.63

DiffDock Confidence

-0.85

Seqdif (SeqID)

69.01 (MEJ5231160)

Structdif (F-Target)

96.51 (5g3y A)

ProtNLM

Name	Score
AK	0.238
Adenylate kinase	0.184
Adenylate monophosphate kinase	0.150
2.7.4.3	0.149
ATP:AMP phosphotransferase	0.141
ATP-AMP transphosphorylase	0.133
ADK_lid domain-containing protein	0.000
Nucleoside monophosphate kinase	0.000
Polyphosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.36 (Lunatic)	18	86.3	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.67 (Lunatic)	33.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.12 (Lunatic)	6	93.7	N/A	N/A
Tierra	T4	Biologics Optimized System	0.16 (Lunatic)	8	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

13_-0.99 | 36