MC3D0

Prompt

This protein has adenylate kinase activity and is involved in the nucleotide metabolic process. It facilitates the conversion of nucleotides within the cell.

AI Generated Sequence

214 AAs | 23.46 kDa | 25.28 kDa (with tag)

MNIILLGPPGAGKGTQAKRLVEKYGIPQISTGDMLRAAVAAGTEVGKKAKAVMDAGELVPDEITIGIVEERIAQDDKKNGFILDGFPRTVAQAEALKEMLADLGKKIDHVINIEVDESVLVERITGRFIEKNTGATYHIEFNPPKVEGVKDKYGGEEFVRRDDDNEETVKTRLEVYRKQTEPLIDFFEAKGLLVNIDGQKDIDKVTADILAVLG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.26

AAComp

89.64

DiffDock Confidence

-1.61

Seqdif (SeqID)

67.29 (WP_003348584)

Structdif (F-Target)

94.82 (4nu0 B)

ProtNLM

Name	Score
AK	0.242
Adenylate kinase	0.187
Adenylate monophosphate kinase	0.151
2.7.4.3	0.144
ATP:AMP phosphotransferase	0.142
ATP-AMP transphosphorylase	0.130
ADK_lid domain-containing protein	0.000
Polyphosphate kinase	0.000
Nucleoside monophosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.14 (Lunatic)	7	96	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.08 (Lunatic)	4	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.07 (Lunatic)	3.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.33 (Lunatic)	16.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	62.9	63

Data

41_-1.00t | 66