MUCG2

Prompt

This protein has RNA helicase activity and is involved in interactions with its host.

AI Generated Sequence

372 AAs | 40.8 kDa | 42.62 kDa (with tag)

MSFESLGLSEKLLKAVADMGYETPTPIQAQAIPQVLEGRDVLGIAQTGTGKTAAFVLPMLQRLSRGRPRARMVRALILAPTRELAAQVAENFETYGKHTKLRSALIFGGVSMNPQIKALERGVDILIATPGRLLDHFERGKIHLSGVEILVLDEADRMLDMGFIPDIEKILKLLPKTRQTLFFSATMPPEIRKLADSFLNNPVRIEVARPASTAETVTQSVVAVDKSQKRELLTHLLRTEGIEQALVFTRTKHGADRLAKQLSRDGISAGAIHGDKDQGARTRALADFRNGDVRILVATDVAARGIDIPGLSHVFNFELPHVPEDYVHRIGRTGRAGRSGEAISLVAPHDMKLLRAIEKLIGQKIPRVEIEG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

72.78

AAComp

94.44

DiffDock Confidence

-1.35

Seqdif (SeqID)

67.47 (WP_206574816)

Structdif (F-Target)

88.49 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.430
ATP-dependent RNA helicase RhlE	0.305
RNA helicase	0.083
ATP-dependent helicase	0.052
3.6.4.13	0.028
ATP-dependent RNA helicase	0.018
Superfamily II DNA/RNA helicase	0.016
Superfamily II DNA and RNA helicase	0.011
DEAD/DEAH box helicase domain protein	0.011
3.6.1.-	0.008

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	3.38 (Lunatic)	169	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	4.72 (Lunatic)	236	100	N/A	N/A
Tierra	T3	Biologics Optimized System	2.79 (Lunatic)	139.5	91.5	N/A	N/A
Tierra	T4	Biologics Optimized System	2.51 (Lunatic)	125.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

104_-2.42t | 106