M3RBZ

Prompt

The protein features RNA helicase activity and is part of the host cell.

AI Generated Sequence

372 AAs | 40.64 kDa | 42.46 kDa (with tag)

MSFSDLGLSEPLLKAVAELGYTEPTPIQAQAIPAVLEGRDVLAGAQTGTGKTAAFSLPILQRLSEGRPRARMVRALILAPTRELAAQVGENFRAYGKHLPLRSLAVFGGVSIGPQIKALERGVDILIATPGRLLDHFEQGKIHLSGVEVLVLDEADRMLDMGFIPDIEKIFKLLPKTRQTLLFSATMPPEIRKLADTFLNNPVRIEVARPNATAETIEQSVVAVDKSRKPELLTHLLRTENLEQVLVFTRTKHGADRLAKQLERDGISAEAIHGDKSQGARTRALADFKSGKVRILVATDVAARGIDIKGLPHVFNYDLPHVPEDYVHRIGRTGRAGRTGEAISLVAADEMHNLRAIEKLIGQKLPRVEIEG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

77.6

AAComp

94.21

DiffDock Confidence

-1.48

Seqdif (SeqID)

69.33 (WP_206574816)

Structdif (F-Target)

87.7 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.391
ATP-dependent RNA helicase RhlE	0.309
RNA helicase	0.123
ATP-dependent helicase	0.041
3.6.4.13	0.029
ATP-dependent RNA helicase	0.018
Superfamily II DNA/RNA helicase	0.016
DEAD/DEAH box helicase domain protein	0.014
Superfamily II DNA and RNA helicase	0.011
DNA/RNA helicase, superfamily II	0.007

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	2.59 (Lunatic)	129.5	94.5	N/A	N/A
Tierra	T4	Enzyme Optimized System	2.95 (Lunatic)	147.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	2.01 (Lunatic)	100.5	96.6	N/A	N/A
Tierra	T4	Biologics Optimized System	2.35 (Lunatic)	117.5	98.7	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	66.5	66.1

Data

94_-1.70t | 104