M1O5J

Prompt

The protein features a RNA helicase activity and a transcription, RNA-templated.

AI Generated Sequence

295 AAs | 32.3 kDa | 34.12 kDa (with tag)

MSFADLGLSEPLLKAVEELGYTTPTPIQAQAIPPVLEGRDVLGIAQTGTGKTAAFTLPILQRLSEGRARPRAQPRALILAPTRELAAQVGENVKAYGKHLPLRSLALIGGVSFNPQIDALKRGVDILIATPGRLLDHYQRGNILFSGVEVLVLDEADRMLDMGFIPDIKKILALVPKTRQTLFFSATMPPEIRKLADTFLNNPVRIEVARPNSTAETIEQSVYPVDHARKPALLSHLIRTENLEQALVFTNTKHDVNRLAKELERDGISAEAIHGDKSQGARLAALEDFKNGRVR

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

78.31

AAComp

94.18

DiffDock Confidence

-1.43

Seqdif (SeqID)

67.12 (WP_152144640)

Structdif (F-Target)

68.06 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.782
ATP-dependent RNA helicase RhlE	0.080
RNA helicase	0.025
ATP-dependent RNA helicase	0.024
DEAD/DEAH box helicase domain protein	0.013
3.6.4.13	0.012
ATP-dependent helicase	0.011
Superfamily II DNA/RNA helicase	0.008
Superfamily II DNA and RNA helicase	0.005
ATP-dependent RNA helicase rhlE	0.005

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.92 (Lunatic)	46	71.8	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.98 (Lunatic)	99	98.5	N/A	N/A
Tierra	T3	Biologics Optimized System	0.44 (Lunatic)	22	73.6	N/A	N/A
Tierra	T4	Biologics Optimized System	0.74 (Lunatic)	37	96.1	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

83adp_-1.03 | 31