MU4X7

Prompt

The protein is involved in the DNA biosynthetic process. It features an ATP-dependent DNA ligase at the C-terminal.

AI Generated Sequence

282 AAs | 29.64 kDa | 31.46 kDa (with tag)

MLFSELAEVSAAVAATSRRLAKTALLAELFRRAEPADVAPVIGFLAGKLRQGRIGVGWKVLAEREEPATAEPPLTVAEVDAALTEIAAVSGPGSQARRRELVAELFARATDAEQRFLFRLLTGELRQGALEAVAADALARAAGVPAERVRRAAMLRGDLPAVAAALLAGGAAALAAVRLTVGRPVRPMLAQPAASVEEALERLGGAAAVEAKLDGARIQVHRAGDEVRLYTRTLDEITARLPELVEAVRALPAERLVLDGETLALDADGRPRPFQETASRVG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

77.16

AAComp

82.93

DiffDock Confidence

-2.28

Seqdif (SeqID)

68.79 (WP_089114442)

Structdif (F-Target)

84.61 (7rpw E)

ProtNLM

Name	Score
ATP-dependent DNA ligase	0.427
DNA ligase	0.253
6.5.1.1	0.228
DNA_LIGASE_A3 domain-containing protein	0.014
DNA ligase (ATP)	0.008
DNA ligase-1	0.007
DNA_ligase_A_N domain-containing protein	0.006
DNA ligase 1	0.004
LIGANc domain-containing protein	0.004
ATP dependent DNA ligase	0.003

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	52.2	54.7

Data

77atp_-1.37t | 74