MR5BT

Prompt

This protein has endoribonuclease activity, generating 5'-phosphomonoesters. It also contains a Helicase superfamily 1/2 ATP-binding domain.

AI Generated Sequence

199 AAs | 21.05 kDa | 22.87 kDa (with tag)

MSLLLSPTRELALQIAEELRALGYPVGLHAGKQSQAERDRLAEFAAGKLRVLVTATVPAGVEALAAGVLREPVHYEVDPAAGLVPELEQHYLRVGDPAKAERLLALLAARGEPRALVFANTKRGADRLAAELAAAGVPVGELHGDLSQPARERVLAEFAAGLDRVLVTTNIEAGRDHPALFARFAAGLDVLVATYHLLG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

67.75

AAComp

85.19

DiffDock Confidence

-2.54

Seqdif (SeqID)

35.09 (HEY5207907)

Structdif (F-Target)

74.86 (6l5n B)

ProtNLM

Name	Score
Helicase C-terminal domain-containing protein	0.261
DEAD/DEAH box helicase	0.196
Helicase	0.067
ATP-dependent helicase	0.060
ATP-dependent DNA helicase RecG	0.054
Helicase conserved C-terminal domain-containin...	0.049
ATP-dependent RNA helicase SrmB	0.041
ATP-dependent DNA helicase	0.022
DNA helicase	0.021
3.6.4.13	0.014

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

89adp_-1.29t | 87