MC272

Prompt

The protein features a Helicase superfamily 1/2 ATP-binding domain involved in the SOS response.

AI Generated Sequence

293 AAs | 30.95 kDa | 32.77 kDa (with tag)

MSLLLSPTRELALQIAEELRRLAPALGLRVAALYGGVDKARQIELLRRGVDVVVATPERLLDHLERGALTLGEVRLLVLDEGHMLDGMGFHPDLERILRLTPPGTQTLMLSATIPPGIEALARRWLRPEIYEVDPPAGELPEGITQHYLAVGDREKARLLCALLDAGEGRALIFVNTRRGAEWLAERLTAAGIPVGVLHGDLSQPERERVLAEFAAGELRVLVTTAIDLGTGRTADLIAALDAGLDAAALAAALAAGLPVGALHGGLSQAERDAVLAAFAAGELRAVLAAPAA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

AAComp

85.43

DiffDock Confidence

-1.66

Seqdif (SeqID)

47.37 (MCX6682465)

Structdif (F-Target)

71.26 (3pey A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.598
ATP-dependent RNA helicase	0.099
Helicase	0.063
RNA helicase	0.029
DEAD/DEAH box helicase domain protein	0.025
3.6.4.13	0.021
Helicase ATP-binding domain-containing protein	0.017
DEAD/DEAH box helicase domain-containing protein	0.013
ATP-dependent helicase	0.012
ATP-dependent RNA helicase SrmB	0.009

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.35 (Lunatic)	17.5	40.6	N/A	N/A
Tierra	T3	Biologics Optimized System	0.21 (Lunatic)	10.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.2 (Lunatic)	10	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	67	71.2

Data

75adp_-1.22 | 99