MX31H

Prompt

This protein is involved in ATP binding, RNA helicase activity, and is crucial for stress response in cells.

AI Generated Sequence

298 AAs | 31.46 kDa | 33.28 kDa (with tag)

MSFADLGLSPALLAAVEAAGYTAPTPIQAQAIPAVLAGRDVLGIAQTGTGKTAAFALPILQRLARPAPRARPQPRALILAPTRELAAQVAENLAALAKHTRLRTLAVFGGVSIGPQIRALERGVDILVATPGRLLDHMARGAVRLDAVETLVLDEADQMLDMGFLHDLRRILALTPKARQTLLFSATMPKEVRRLAGTFLRDPVEVQVAPAAAPAETVTQWVVPVGDRDKPALLTHLLAGGAPERALVFTRTKHGANRVAKHLEAAGIPAAALHGGKSQGARTRALAAFRDGRVRAVA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

71.24

AAComp

86.12

DiffDock Confidence

-1.59

Seqdif (SeqID)

59.6 (NRA72102)

Structdif (F-Target)

67.25 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.640
RNA helicase	0.154
ATP-dependent RNA helicase	0.049
ATP-dependent RNA helicase RhlE	0.045
Superfamily II DNA/RNA helicase	0.024
DEAD/DEAH box helicase domain protein	0.015
3.6.4.13	0.014
ATP-dependent helicase	0.012
Superfamily II DNA and RNA helicase	0.009
Helicase	0.003

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	2.27 (Lunatic)	113.5	1.7	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.51 (Lunatic)	25.5	53.8	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

74atp_-1.22 | 45