MIY6R

Prompt

This protein focuses on breaking down inositol phosphates and modifying phosphatidylinositol lipids in cucumber.

AI Generated Sequence

289 AAs | 32.61 kDa | 34.43 kDa (with tag)

KRVGYALTEKKIKNIQPQAFLDLARKRGIELVQVDLSRPLEDQGPFDVIVHKLTDPIEQQVHEYIDAHPETVVLDPPDAIHTLLDRSQMLELIKDLDLSMEDGRVCVPPMEVIKKNLTFPLICKPLVAHGTNSHEMALIFHDEGLSAIQPPLVLQNFINHGGVLYKVFVVGESITVVQRRSLPNFSAGLEDRNVFFNSHSVSKPESSSVRLTALDDLDPRIERPPSDELLRELAKGLRQALGLSLFNFDIIIENQTGQHAVIDINFFPGFEGMPEFFEHLVDHFLNLVQ

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

50.26

AAComp

91.76

DiffDock Confidence

-0.41

Seqdif (SeqID)

61.71 (XP_051833316)

Structdif (F-Target)

90.78 (2odt X)

ProtNLM

Name	Score
Inositol-tetrakisphosphate 1-kinase	0.543
2.7.1.159	0.180
2.7.1.134	0.172
ITPK1 kinase	0.034
Inositol-1,3,4-trisphosphate 5/6-kinase	0.007
Inositol 1,3,4-trisphosphate 5/6-kinase	0.006
Ins134_P3_kin domain-containing protein	0.003
ATP-grasp domain-containing protein	0.002
2.7.1.59	0.001
Inositol-tetrakisphosphate 1-kinase 1	0.001

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.11 (Lunatic)	5.5	61.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.01 (Lunatic)	0.5	47.3	N/A	N/A
Tierra	T3	Biologics Optimized System	0.31 (Lunatic)	15.5	74.2	N/A	N/A
Tierra	T4	Biologics Optimized System	0.1 (Lunatic)	5	64.7	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

20_-1.00t | 71