M8SCR

Prompt

The protein features a CDC48 domain 2-like superfamily and a Vps4 oligomerisation, C-terminal.

AI Generated Sequence

296 AAs | 32.88 kDa | 34.7 kDa (with tag)

MADDLSTAILEQKKRANELIVDEAVNEDNSVVAMSPKEMEKLQRFRGDTVLVKGKNRRETVCIVLADDEKDDEPIRMNRVQRNNLRVRLGDIISIQPCPDVKAGKRIHVLPIDDTVEGVTGNLFDVYLKPRLLEAYRPVRKGDLFIVRGGMRAVEFKVVEADPSPHGIVAPDTVIHNEGEPIKRDEERENLEEVGVDDIGGVRKQLAQIREMVELPLRHPQLFDSIGIRPPRGVLLFGPPGTGKTLIARAVANETGAFFFLINAPEIMSKAAGESEANLRKVFEEAENASPADFLG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

60.85

AAComp

91.79

DiffDock Confidence

-1.74

Seqdif (SeqID)

78.77 (XP_010770092)

Structdif (F-Target)

55.3 (3qc8 A)

ProtNLM

Name	Score
AAA family ATPase	0.613
CDC48	0.027
3.6.4.6	0.025
CDC48 family AAA ATPase	0.021
CDC48/PAS1/SEC18 family ATPase	0.016
Cell division cycle protein 48 homolog	0.015
VCP-like ATPase	0.015
Proteasome-activating nucleotidase	0.010
Transitional endoplasmic reticulum ATPase	0.010
CDC48/PAS1/SEC18 family of ATPases	0.009

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.49 (Lunatic)	24.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.18 (Lunatic)	9	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.22 (Lunatic)	11	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	73.8	73.8

Data

80_-1.42 | 103