MBZMQ

Prompt

This protein is a P-type ATPase that transports calcium ions. It uses ATP to move calcium across membranes.

AI Generated Sequence

287 AAs | 29.75 kDa | 31.57 kDa (with tag)

MKEAVVRRDGTEVEVPAEQVVVGDIIEVRPGDKIPVDGVVVEGHSEVNESMLTGESLPVEKKVGDKVMAGTINGEGAFEVEATKVGTDTVAAKIVELVKDAQTDKSEIQRLIDKIAKFYTPVLIIPAGLTAIWQSLGLDFKTALVTVVAVLVAICPDGLGLTPTMAFMIGAGRALKQGVFNIAVDGTGNIPAADAIALGDLIGTGTNVAIGDGTILKDGTPVKDAPALVKLLGDNVLMGDNVAKATALDKLMALAPEKAHVLRDGTEVVDVPAEQLVVGDIIRVRPG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

64.62

AAComp

88.54

DiffDock Confidence

-1.74

Seqdif (SeqID)

44.3 (CZE07519)

Structdif (F-Target)

35.18 (2voy F)

ProtNLM

Name	Score
HAD-IC family P-type ATPase	0.096
Haloacid dehalogenase-like hydrolase	0.094
HAD family hydrolase	0.087
3.6.3.-	0.071
Cation-translocating P-type ATPase	0.067
P-type E1-E2 ATPase	0.066
Copper-translocating P-type ATPase	0.059
3.6.3.3	0.033
HAD ATPase, P-type, family IC	0.027
Lead, cadmium, zinc and mercury transporting A...	0.024

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.34 (Lunatic)	17	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	0.14 (Lunatic)	7	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.31 (Lunatic)	15.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

86adp_-1.17t | 32