MJ0WZ

Prompt

This protein is part of the adenylate kinase family and has nucleobase-containing compound kinase activity. It facilitates the phosphorylation of nucleobase compounds.

AI Generated Sequence

214 AAs | 23.1 kDa | 24.92 kDa (with tag)

MRIVLLGPPGAGKGTQARRLAEEFGIVQISTGDMLRANVAAGTELGKEAKAVMAAGGLVPDEIVIGIVRERLAEEDEAKGFIFDGFPRNVAQAEALGEMLAERGLKLDAVLEFEVDEEVMVERISGRIVTASPGAGNHELFKRPKEEGVDDKKGGEIFKRDDDKNEETVKARLEELRKKTEPLLDFYEAKGLLSNIDGLAEIDAVFADVEAALG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

68.87

AAComp

88.54

DiffDock Confidence

-1.51

Seqdif (SeqID)

61.03 (HEX9771425)

Structdif (F-Target)

95.22 (1p3j A)

ProtNLM

Name	Score
AK	0.238
Adenylate kinase	0.188
2.7.4.3	0.152
Adenylate monophosphate kinase	0.147
ATP:AMP phosphotransferase	0.143
ATP-AMP transphosphorylase	0.128
ADK_lid domain-containing protein	0.000
Nucleoside monophosphate kinase	0.000
Polyphosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.15 (Lunatic)	7.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.55 (Lunatic)	77.5	74.7	N/A	N/A
Tierra	T3	Biologics Optimized System	0.5 (Lunatic)	25	100	N/A	N/A
Tierra	T4	Biologics Optimized System	1.33 (Lunatic)	66.5	84.9	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

64_-1.27t | 50