M77O8

Prompt

This protein is a RNA helicase from Ord's kangaroo rat, involved in RNA splicing and metabolic processes.

AI Generated Sequence

297 AAs | 32 kDa | 33.82 kDa (with tag)
MSFADLGLSPALLRAVEELGYTEPTPIQAQAIPAVLAGRDVLGLAQTGTGKTAAFALPILQRLSRPAPRARPQPRALILAPTRELAAQVAENLAAYAKHLPLRTLAVFGGVSIGPQIKALERGVDILVATPGRLLDHVERGALRLDRVETLVLDEADRMLDMGFIHAVRRILALTPRERQTLLFSATMPKAVRDLAGRFLRDPVEVEVAPRSTTADTVEQWVVPVAKQDKRELLTHLLARGPGVRALVFTRTKHGADRLAKHLERAGIAAAAIHGGKSQGARTRALADFKAGRVRAV

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

96

GPT Score

73.64

AAComp

88.52

DiffDock Confidence

-1.5

Seqdif (SeqID)

66.67 (WP_009151138)

Structdif (F-Target)

67.8 (4kbg A)

ProtNLM

NameScore
DEAD/DEAH box helicase0.708
RNA helicase0.152
ATP-dependent RNA helicase RhlE0.034
ATP-dependent RNA helicase0.029
DEAD/DEAH box helicase domain protein0.024
ATP-dependent helicase0.012
Superfamily II DNA/RNA helicase0.011
DEAD/DEAH box helicase domain-containing protein0.005
Superfamily II DNA and RNA helicase0.004
3.6.4.130.004

Experimental Results

CompanyVariantExpression SystemConcentration (mg/ml)Total Yield (µg)Purity (%)TM (°C)Tm(oC w/2mM AMP-PNP)
TierraT3Enzyme Optimized System0.58 (Lunatic)2968.1N/AN/A
TierraT4Enzyme Optimized SystemN/AN/AN/AN/AN/A
TierraT3Biologics Optimized SystemN/AN/AN/AN/AN/A
TierraT4Biologics Optimized SystemN/AN/AN/AN/AN/A
ArctorisN/AE. coliN/AN/AN/Alow confidence66.4

Data

98adp_-1.40 | 68
Tierra Image 1Tierra Image 2Arctoris Image 1
Designed by MP, using an AMD MI250 GPU