MEM9A

Prompt

This protein has ATP-dependent activity and functions on DNA. It is involved in DNA replication.

AI Generated Sequence

222 AAs | 23.91 kDa | 25.73 kDa (with tag)

MLEVRNVSKRYGDREVLDGVSFTVPAGEIVALVGPSGSGKSTLLRLIAGLEEPTSGEIVVDGRDITGLPPAERRRIGYVFQHNLLFPNVLTVRDNVAFGARLPGLPRAERDARVAELLARVDLDALADRPIAELSGGEQQRVALARALVAEPRLLLLDEPTANLDPVLRRQLLAELRALLAERGTTVVVTTHDLAVAELADRVVVMEDGRIVAQGTPAELFA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

67.66

AAComp

88.44

DiffDock Confidence

-0.82

Seqdif (SeqID)

54.3 (MBE0454122)

Structdif (F-Target)

92.74 (2pcj B)

ProtNLM

Name	Score
ABC transporter ATP-binding protein	0.356
ATP-binding cassette domain-containing protein	0.246
ABC transport system ATP-binding protein	0.058
Amino acid ABC transporter ATP-binding protein	0.038
Glutamine transport ATP-binding protein GlnQ	0.034
ABC transporter domain-containing protein	0.030
Peptide ABC transporter ATP-binding protein	0.021
Amino acid ABC transporter ATP-binding protein...	0.011
ABC transporter related protein	0.011
ABC transporter related	0.009

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.72 (Lunatic)	36	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.07 (Lunatic)	53.5	93.9	N/A	N/A
Tierra	T3	Biologics Optimized System	0.12 (Lunatic)	6	86.2	N/A	N/A
Tierra	T4	Biologics Optimized System	0.46 (Lunatic)	23	98.7	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

1atp_-0.90t | 3