M7QCU

Prompt

The protein functions as an arsenical pump ATPase and is involved in the ArsA/GET3 pathway. It plays a role in cellular protein localization.

AI Generated Sequence

240 AAs | 25.88 kDa | 27.7 kDa (with tag)

MSFEKLLDIDTKRYLFFTGKGGVGKTSIAAATALALAEAGKKVLLVSTDPASNLGQVFDVQIGNKIEPVKGVPNLTALNIDPQEIAAEYREAVVPDIRGLLPEEVLAEMKEQLSGASTTEIAAFDEFTAFLTDEALKGEFDHVIFDTAPTGHTLRMLQLGPALSNFLEDNNGPSAKLGPLPGLLEQKKKIQEAVHALSDQTETKLIVLNRPELSKLQETERAAEELKKIGIENQALIVNG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

78.6

AAComp

92.12

DiffDock Confidence

-1.54

Seqdif (SeqID)

60.79 (PLP44175)

Structdif (F-Target)

80.2 (3zq6 B)

ProtNLM

Name	Score
Arsenical pump-driving ATPase	0.311
Arsenic-transporting ATPase	0.162
ArsA_ATPase domain-containing protein	0.132
Arsenite efflux ATP-binding protein ArsA	0.076
Arsenite-transporting ATPase	0.061
Arsenic ABC transporter ATPase	0.048
Arsenite-activated ATPase ArsA	0.047
3.6.3.16	0.038
Arsenic transporter ATPase	0.014
Anion-transporting ATPase	0.009

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.44 (Lunatic)	22	95.6	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.43 (Lunatic)	71.5	96.9	N/A	N/A
Tierra	T3	Biologics Optimized System	0.67 (Lunatic)	33.5	94.3	N/A	N/A
Tierra	T4	Biologics Optimized System	0.45 (Lunatic)	22.5	98.6	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

49_-1.12t | 53