M50VT

Prompt

The protein features a hydrogen sulfide metabolic process and a sulfur amino acid metabolic process.

AI Generated Sequence

197 AAs | 21.86 kDa | 23.68 kDa (with tag)

MSTNIVWHAHPVTKAERAALNGHRGFVVWFTGLSGSGKSTIANALEKQLFELGRHTYRLDGDNVRHGLNKDLGFSEADRVENIRRIGEVAKLMADAGLIVLTAFISPYREDRDMVRELLPEGDFIEVFVDTPLAVCEARDPKGLYKKARAGEIKHFTGISAVYEAPENAEIVLDTGELTVEESVAQVIDYLKREGII

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

56.14

AAComp

93.78

DiffDock Confidence

-1.0

Seqdif (SeqID)

68.02 (WP_372761269)

Structdif (F-Target)

92.06 (3uie C)

ProtNLM

Name	Score
APS kinase	0.268
Adenylyl-sulfate kinase	0.202
2.7.1.25	0.191
Adenosine-5'-phosphosulfate kinase	0.170
ATP adenosine-5'-phosphosulfate 3'-phosphotran...	0.163
Adenylylsulfate kinase	0.001
2.7.7.4	0.001
Sulfate adenylate transferase	0.000
Multifunctional fusion protein	0.000
Sulfate adenylyltransferase subunit CysN	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.16 (Lunatic)	8	89.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.31 (Lunatic)	15.5	81.2	N/A	N/A
Tierra	T3	Biologics Optimized System	0.12 (Lunatic)	6	88.6	N/A	N/A
Tierra	T4	Biologics Optimized System	0.07 (Lunatic)	3.5	91.8	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	55.3	55.2

Data

48atp_-0.64t | 61