M3Y6D

Prompt

The protein features a RNA helicase activity and a viral process.

AI Generated Sequence

369 AAs | 40.53 kDa | 42.35 kDa (with tag)

MSFSDLGLSEPLLKAVEELGYTKPTPIQAQAIPAILEGRDVLAIAQTGTGKTAAFVLPILQRLSSGRPRRRQVRALVLTPTRELAAQVAENVRTFGKFLKLRSVAVIGGVSIGPQIDKLERGVDILVATPGRLLDHMERGKISLSGVEILVLDEADRMLDMGFIHDIKKILSLLPKERQTLLFSATMPPEIRTLAQKFLHDPVRIEVAPRTTTAETIEQAVVPVDKQRKTELLSHLLRTGNMEQVLVFTNTKRGADELAKQLERDGISAAAIHGDKSQGQRLKALSDFRSGKVRVLVATDVAARGLDIKGLPHVINFDLPHDPEDYVHRIGRTGRAGRSGEAITLVAADEMHRLNAIERLIGQKLPRKE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

76.68

AAComp

93.78

DiffDock Confidence

-1.42

Seqdif (SeqID)

68.31 (WP_152144640)

Structdif (F-Target)

88.75 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.339
ATP-dependent RNA helicase RhlE	0.290
RNA helicase	0.154
ATP-dependent helicase	0.058
ATP-dependent RNA helicase	0.046
3.6.4.13	0.036
DEAD/DEAH box helicase domain protein	0.015
Superfamily II DNA/RNA helicase	0.012
Superfamily II DNA and RNA helicase	0.008
ATP-dependent RNA helicase rhlE	0.005

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	7.62 (Lunatic)	381	92.7	N/A	N/A
Tierra	T4	Enzyme Optimized System	8.94 (Lunatic)	447	100	N/A	N/A
Tierra	T3	Biologics Optimized System	8.77 (Lunatic)	438.5	82.2	N/A	N/A
Tierra	T4	Biologics Optimized System	7.39 (Lunatic)	369.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

33_-1.00 | 33