M2VZK

Prompt

The protein has RNA helicase activity and is associated with the Porcine reproductive and respiratory syndrome virus (PRRSV). It helps in unwinding RNA structures during the viral replication process.

AI Generated Sequence

296 AAs | 32.48 kDa | 34.3 kDa (with tag)

MTFADLGLSEPLLAAVEEAGFTEPTPIQAQAIPLALEGRDVLGRAQTGTGKTAAFSLPILQRLDRPAPRPQRALILAPTRELAIQIQEALADLGRHRRLRVLAVYGGADIGRQIRALRRGAHIVVATPGRLLDHLRRGTLRLDGVETLVLDEADQMLDMGFLPDVRRIAAAVPAERQTLLFSATMPPAVKRIGERFLRDPVEVRVAPAAATVPEITQRYVLVEDAEKFAVLTRLLDAAQPERVIVFTRTKHRVDELAKALTARGFRAEGLHGDKSQGARLRVLERFRSGRLRAVVA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

69.06

AAComp

87.97

DiffDock Confidence

-1.16

Seqdif (SeqID)

60.81 (WP_207694669)

Structdif (F-Target)

67.29 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.726
RNA helicase	0.115
ATP-dependent RNA helicase	0.045
DEAD/DEAH box helicase domain protein	0.031
ATP-dependent helicase	0.029
ATP-dependent RNA helicase RhlE	0.009
DEAD/DEAH box helicase domain-containing protein	0.009
DEAD-box ATP-dependent RNA helicase CshA	0.005
3.6.4.13	0.005
Superfamily II DNA/RNA helicase	0.005

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

84atp_-1.26 | 20