M0VAG

Prompt

This protein contains a Helicase superfamily 1/2 ATP-binding domain of the DinG/Rad3-type. It also exhibits DNA polymerase activity.

AI Generated Sequence

288 AAs | 31.54 kDa | 33.36 kDa (with tag)

MSLSELLERLRERGFEELTPIQAEAIPAILEGRDVVAVAPTGSGKTLAFELPMLQRLAAGERALVLSPTRELANQIAAELRALAPYLGLRVAAVYGGVDKARQIELLREGVQIVVATPGRLLDHLERGALRLGEVRFLVLDEGDQMLDMGFHPDVERILRLTPPGERQLMFSATIPGGVERLARRWLREPVEIEVDPPAELPEGITQHYFAVDDREKARLLVALLEAGEGGRALVFVNTKRGAERLAAWLTEAGIPVAELHGGLSQPERERVLAGFRAGELRVLVTTA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

67.47

AAComp

87.82

DiffDock Confidence

-0.92

Seqdif (SeqID)

50.68 (OGO66868)

Structdif (F-Target)

65.29 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.705
ATP-dependent RNA helicase	0.061
DEAD/DEAH box helicase domain protein	0.047
DEAD-box ATP-dependent RNA helicase CshA	0.025
DEAD/DEAH box helicase domain-containing protein	0.020
ATP-dependent helicase	0.019
RNA helicase	0.018
Helicase	0.013
Superfamily II DNA/RNA helicase	0.009
3.6.4.13	0.008

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.26 (Lunatic)	13	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	0.21 (Lunatic)	10.5	72.8	N/A	N/A
Tierra	T4	Biologics Optimized System	0.22 (Lunatic)	11	44.4	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

52atp_-0.91 | 35