M358F

Prompt

The protein features an AAA C-terminal domain involved in DNA recombination.

AI Generated Sequence

292 AAs | 32.49 kDa | 34.31 kDa (with tag)

MSDLFGADDAPLADRLRPRTLDEVVGQDHLLGEDGPLRRMLESGRLSSMIFWGPPGTGKTTLARLLAGASDLHFEQISAVFSGVADLKKVFEAARQRRAMGQRTLLFVDEIHRFNRAQQDAFLPHVEDGTVTLVGATTENPSFELNSALLSRAQVYVLNALDEAALTRLLERAEETERGLGKLPLDEEARALLIAMADGDGRFLLNLLEQLWAARPEEVFDTAGLAERVQRRAPRYDKSQDEHYNLISALHKSVRGSDPDAALYWLARMLDAGEDPRYLARRLVRMASEDIG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

70.28

AAComp

90.35

DiffDock Confidence

-0.87

Seqdif (SeqID)

74.75 (MCO5084112)

Structdif (F-Target)

65.92 (2chg C)

ProtNLM

Name	Score
Replication-associated recombination protein A	0.748
AAA family ATPase	0.153
Replication-associated recombination protein RarA	0.041
Recombination factor protein RarA	0.018
AAA domain-containing protein	0.013
ATPase AAA	0.004
ATPase	0.003
ATPase, AAA family	0.002
AAA ATPase central domain protein	0.001
Recombination protein RarA	0.001

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.16 (Lunatic)	8	93.3	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.23 (Lunatic)	11.5	99.1	N/A	N/A
Tierra	T3	Biologics Optimized System	0.02 (Lunatic)	1	88.6	N/A	N/A
Tierra	T4	Biologics Optimized System	0.01 (Lunatic)	0.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

43atp_-0.65t | 41