MTZB5

Prompt

Adenylate kinase is involved in the nucleotide biosynthetic process. It contains a conserved site essential for its function.

AI Generated Sequence

189 AAs | 21.02 kDa | 22.84 kDa (with tag)

MPRFVVVTGIGPGVGKTTLVNEALARETGERYKGINFGNVMFEEAAKEGLVAHRDEMRKLPLETQRQLQKQAAGRIVALAREGPVVLDTHASIKTPSGYLPGLPPEVLERLNPDLVVVIEADPQEILGRRLRDETRHRDIETEEQIERHEQMNRAASMAYAVMTGAVIKIVENRDGLKEEAVQELLAEL

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.38

AAComp

90.27

DiffDock Confidence

-0.58

Seqdif (SeqID)

61.17 (WP_013747832)

Structdif (F-Target)

96.54 (3h86 6)

ProtNLM

Name	Score
AK	0.321
Adenylate kinase	0.262
2.7.4.3	0.217
ATP-AMP transphosphorylase	0.193
Adenylate monophosphate kinase	0.001
AAA family ATPase	0.001
ATP:AMP phosphotransferase	0.000
Nucleoside monophosphate kinase	0.000
ADK_lid domain-containing protein	0.000
Dual activity adenylate kinase/ATPase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.25 (Lunatic)	12.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.4 (Lunatic)	20	83.6	N/A	N/A
Tierra	T3	Biologics Optimized System	0.29 (Lunatic)	14.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.03 (Lunatic)	1.5	86.6	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

47atp_-0.87 | 28