MAKA5

Prompt

The protein features a AAA ATPase, AAA+ lid domain and a Vps4 oligomerisation, C-terminal.

AI Generated Sequence

274 AAs | 30.71 kDa | 32.53 kDa (with tag)

TPAELIVDEAVNEDNSVLVMNAKEMDKLQRFEGRDVAIKGKNRRDTICIVLSDDEKDDEPIRMNRVQRNNLRVRLDDIISIQPCPDVKYAKRIHVLPIDDITEGVGGNLDGVFLKQYLEAARRPVRKDDTLFVRGGMGAVEFVKVIEPPKGIYIVQPDTVFHIGGEPIKREDEENNLDEVGVDDIGGVRQELAQIREMVELPLRHPQLFDKIGVRPPRGVLLFGPPGTGKTLLARAVANETGAFFFLINAPEIMSKLSGESEENLRKVFEEADN

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

69.37

AAComp

90.48

DiffDock Confidence

-1.33

Seqdif (SeqID)

69.85 (XP_010770092)

Structdif (F-Target)

56.89 (3qc8 A)

ProtNLM

Name	Score
AAA family ATPase	0.603
CDC48_2 domain-containing protein	0.100
CDC48 family AAA ATPase	0.045
Transitional endoplasmic reticulum ATPase	0.025
ATPase_AAA_core domain-containing protein	0.021
3.6.4.6	0.018
Cell division cycle protein	0.013
Cell division cycle protein 48	0.012
VCP-like ATPase	0.011
CDC48 domain-containing protein	0.010

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	0.64 (Lunatic)	32	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.91 (Lunatic)	45.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

101atp_-0.83t | 67