MKUGO

Prompt

This protein is involved in RNA processing, binds ATP, and has helicase activity to unwind RNA structures.

AI Generated Sequence

172 AAs | 19.17 kDa | 20.99 kDa (with tag)

MLAALRERGLRLGRLRLLVLDEADTLSDFGFHDDVRRILALAPEAQQVIFSATPVGEIERLVAPTLRPHLGIDVDPSARGLTPQIYQHVELGSKKEKDRLLALIARLQPGQTALIFANTRRVADELHEELRRAGFHVGYLHGDKSQKAERDALAEFQAGEWRVLLVTATEIG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

75.96

AAComp

90.08

DiffDock Confidence

-2.55

Seqdif (SeqID)

41.51 (MCL1855549)

Structdif (F-Target)

75 (8oos G)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.162
3.6.4.13	0.104
RNA helicase	0.085
Helicase	0.084
Helicase C-terminal domain-containing protein	0.081
ATP-dependent RNA helicase SrmB	0.076
ATP-dependent RNA helicase	0.055
Helicase conserved C-terminal domain-containin...	0.038
ATP-dependent RNA helicase DbpA	0.029
ATP-dependent helicase	0.025

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.17 (Lunatic)	8.5	61.9	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.1 (Lunatic)	5	49.4	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

93atp_-1.43t | 72