MZSHY

Prompt

This protein has RNA helicase activity and is involved in interactions with its host.

AI Generated Sequence

372 AAs | 40.86 kDa | 42.68 kDa (with tag)

MSFESLGLSEKLLKAVADMGYETPTPIQAQAIPQVLEGRDVLGIAQTGTGKTAAFVLPMLQRLSRGRPRARMVRALILAPTRELAAQVAENFETYGKYTKLRSALIFGGVSMNPQIKALERGVDILIATPGRLLDHFERGKIHLSGVEILVLDEADRMLDMGFIPDIEKICKLLPKTRQTLFFSATMPPEIRKLADSFLNNPVRIEVARPASTAETVTQSVVAVDKSQKRELLTHLLRTEGIEQALVFTRTKHGADRLAKQLSRDGISAGAIHGDKDQGARTRALADFRNGDVRILVATDVAARGIDIPGLSHVFNFELPHVPEDYVHRIGRTGRAGRSGEAISLVAPHDMKYLRAIEKLIGQKIPRVEIEG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

72.78

AAComp

94.8

DiffDock Confidence

-1.5

Seqdif (SeqID)

66.94 (WP_409668340)

Structdif (F-Target)

88.37 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.518
ATP-dependent RNA helicase RhlE	0.234
RNA helicase	0.080
ATP-dependent helicase	0.047
3.6.4.13	0.024
DEAD/DEAH box helicase domain protein	0.015
Superfamily II DNA/RNA helicase	0.013
ATP-dependent RNA helicase	0.013
Superfamily II DNA and RNA helicase	0.012
3.6.1.-	0.007

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	2.59 (Lunatic)	129.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	2.99 (Lunatic)	149.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	1.76 (Lunatic)	88	100	N/A	N/A
Tierra	T4	Biologics Optimized System	2.02 (Lunatic)	101	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	52.5	53.8

Data

104adp_-1.49t | 98