MS4BB

Prompt

The protein features a nucleoside biosynthetic process and a AMP salvage.

AI Generated Sequence

188 AAs | 20.09 kDa | 21.91 kDa (with tag)

MNIVFLGPPGAGKGTQAARLAEKFGLPHLSTGDLLRAEVAAGTELGKQAKAIMDAGELVPDELVNGMVAERIAQPDAKGGFILDGYPRTVPQAEALDAMLAKLGQKLDAVVEFEVDESTLVERVLGRAKTSGRADDNEETFKARLEVYHAETAPVIDYYKAQGKLHAVDGMGSVEEVFARMQALLDKL

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

56.93

AAComp

90.72

DiffDock Confidence

-1.11

Seqdif (SeqID)

62.03 (WP_339448043)

Structdif (F-Target)

94.43 (2cdn A)

ProtNLM

Name	Score
AK	0.239
Adenylate kinase	0.189
Adenylate monophosphate kinase	0.145
2.7.4.3	0.145
ATP:AMP phosphotransferase	0.141
ATP-AMP transphosphorylase	0.136
ADK_lid domain-containing protein	0.000
Nucleoside monophosphate kinase	0.000
Polyphosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.72 (Lunatic)	36	97.9	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.07 (Lunatic)	3.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.01 (Lunatic)	0.5	95.9	N/A	N/A
Tierra	T4	Biologics Optimized System	0.13 (Lunatic)	6.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

10atp_-0.93 | 40