MKE6W

Prompt

The protein contains a DEAD/DEAH box helicase domain and a TRCF-like, C-terminal D7 domain. These domains are crucial for its function.

AI Generated Sequence

275 AAs | 29.44 kDa | 31.26 kDa (with tag)

MSFADLGLSPALLAAVEAAGYTAPTPIQAQAIPHVLEGRDVLGLAQTGTGKTAAFGLPILQRLARGRARPARPVRALILAPTRELAAQIAENLAAYGKHLPLRTLAVFGGVSINPQIKALERGVDILVATPGRLLDHFERGAVKLDQVEILVLDEADQMLDMGFIHPIRKILKLTPKTRQTLLFSATMPKEIRKLAEGFLHNPVRVEVSPPSATAEKVTQWVVPVAKSQKPALLSHLLAGEPLERALVFTRTKHGADKLAKHLERAGIAAGAIHG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

62.93

AAComp

90.71

DiffDock Confidence

-1.58

Seqdif (SeqID)

62.55 (NRA72102)

Structdif (F-Target)

73.26 (4kbg A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.799
ATP-dependent RNA helicase RhlE	0.058
RNA helicase	0.039
ATP-dependent RNA helicase	0.020
DEAD/DEAH box helicase domain protein	0.013
Helicase ATP-binding domain-containing protein	0.012
3.6.4.13	0.010
ATP-dependent helicase	0.009
Superfamily II DNA/RNA helicase	0.007
3.6.1.-	0.003

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	1.9 (Lunatic)	95	50	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	2.2 (Lunatic)	110	78.1	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

73atp_-1.42t | 86