MOFBD

Prompt

The protein has a Hint domain at the N-terminal and exhibits helicase activity. These features are crucial for its function.

AI Generated Sequence

226 AAs | 24 kDa | 25.82 kDa (with tag)
MSLLLSPTRELALQIAEELRRLAPALGLRVAALYGGVDKAREIALLRDGVDVVVATPGRLLDHLERGALALGQVRLLVLDEGHMLDGMGFHPDLEAILRLTPPGTQTLMLSATIPPGVEALARRYLRPEIVHVDPPAGELPEGITQHVLAVGDREKARLLAALLDAGEGRALIFVNTKRGAERLAERLAAAGIPVGVLHGGLSQPEREAVLAEFAAGELRVLVTTA

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

93

GPT Score

70.9

AAComp

86.43

DiffDock Confidence

-1.12

Seqdif (SeqID)

46.64 (XCI63457)

Structdif (F-Target)

82.52 (7oi6 x)

ProtNLM

NameScore
DEAD/DEAH box helicase0.621
ATP-dependent RNA helicase0.103
Helicase ATP-binding domain-containing protein0.043
RNA helicase0.041
DEAD/DEAH box helicase domain protein0.025
Helicase0.021
ATP-dependent RNA helicase DbpA0.017
ATP-dependent helicase0.017
3.6.4.130.015
DEAD/DEAH box helicase domain-containing protein0.012

Experimental Results

CompanyVariantExpression SystemConcentration (mg/ml)Total Yield (µg)Purity (%)TM (°C)Tm(oC w/2mM AMP-PNP)
TierraT3Enzyme Optimized SystemN/AN/AN/AN/AN/A
TierraT4Enzyme Optimized SystemN/AN/AN/AN/AN/A
TierraT3Biologics Optimized SystemN/AN/AN/AN/AN/A
TierraT4Biologics Optimized SystemN/AN/AN/AN/AN/A
ArctorisN/AE. coliN/AN/AN/AN/AN/A

Data

88_-1.57t | 82
Tierra Image 1Tierra Image 2Arctoris Image 1
Designed by MP, using an AMD MI250 GPU