MN3VY

Prompt

The protein has a Hint domain at the N-terminal and exhibits helicase activity. These features are crucial for its function.

AI Generated Sequence

225 AAs | 24.17 kDa | 25.99 kDa (with tag)

MSLLLSPTRELALQIAEELRRLAPALGLRVAALYGGVDKAREIALLRDGVDVVVATPGRLLDHLERGALALGQVRLLVLDEGHMLDGMGFHPDLEAILRLTPPGTQTLMLSATIPPGVEALARRWLRPEIYHVDPPAGELPEGITQHYLAVGDREKARLLCALLDAGEGRALIFVNTKRGAEWLAERLCAAGIPVGVLHGGLSQPEREAVLAEFAAGELRVLVTT

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

70.9

AAComp

87.07

DiffDock Confidence

-1.9

Seqdif (SeqID)

46.02 (MCX6682465)

Structdif (F-Target)

82.62 (7oi6 x)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.576
ATP-dependent RNA helicase	0.108
Helicase ATP-binding domain-containing protein	0.057
Helicase	0.043
RNA helicase	0.028
ATP-dependent helicase	0.024
ATP-dependent RNA helicase DbpA	0.021
DEAD/DEAH box helicase domain protein	0.019
3.6.4.13	0.019
DEAD/DEAH box helicase domain-containing protein	0.014

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	75.4	77.4

Data

88adp_-1.29t | 102