M7U37

Prompt

The protein contains a PRD domain and an RNA polymerase sigma factor 54 interaction domain. These domains facilitate specific protein functions and interactions.

AI Generated Sequence

248 AAs | 27.11 kDa | 28.93 kDa (with tag)
MSELERLRERLAATLADPLAATLAEVERAAAVDVSTIIGRSTGVGKELAARAHRDLSPRARGPFVAVNCAALPESLFESLFGHERAGSFTGAERQRIGLLEALAGGTLFLDEIGDMPLSLQAKLLRVLEERGVRRVGGRTPIEVDVRVIAATNRDLEAAVRAGRFREDLFYRLNVVPLHLPPLRERREDIPLLVDHFLAEFARRRGRPVPSLSAEALAALRAHPWPGNVRELENVIERAAVLAGGEVE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

92

GPT Score

66.13

AAComp

87.02

DiffDock Confidence

-0.72

Seqdif (SeqID)

58.37 (NPB09826)

Structdif (F-Target)

89.47 (6luf D)

ProtNLM

NameScore
Sigma-54-dependent Fis family transcriptional ...0.369
Sigma-54 factor interaction domain-containing ...0.326
Fis family transcriptional regulator0.076
Transcriptional regulatory protein ZraR0.026
Sigma54 specific transcriptional regulator, Fi...0.015
Transcriptional regulator0.010
DNA-binding response regulator0.010
Response regulator0.009
Two component, sigma54 specific, transcription...0.009
Response regulator of zinc sigma-54-dependent ...0.008

Experimental Results

CompanyVariantExpression SystemConcentration (mg/ml)Total Yield (µg)Purity (%)TM (°C)Tm(oC w/2mM AMP-PNP)
TierraT3Enzyme Optimized SystemN/AN/AN/AN/AN/A
TierraT4Enzyme Optimized SystemN/AN/AN/AN/AN/A
TierraT3Biologics Optimized SystemN/AN/AN/AN/AN/A
TierraT4Biologics Optimized SystemN/AN/AN/AN/AN/A
ArctorisN/AE. coli0.049 (A660nm)6.37>95low confidencelow confidence

Data

0_-0.58t | 5
Tierra Image 1Tierra Image 2Arctoris Image 1
Designed by MP, using an AMD MI250 GPU