MCWDA

Prompt

The protein features a carboxylic acid transmembrane transporter activity and a nitrogen compound transport.

AI Generated Sequence

239 AAs | 26.52 kDa | 28.34 kDa (with tag)

MIELKNLNKWFGDFHVLKGIDLKVPKGEKVVIIGPSGSGKSTLLRCINGLEEPTSGQIVFDGEKLGKPGKNLKRVRNLMGFFQHFELFPHLTVLENVTLAPIKVLGLPKKEEEERAMRLLEVGGIRDHANKYPGQLSGGQQQRIAIARALAMEPEIMLFDEPTSALDPELVGEVLDVIRNLVNSGMTMLVTVHEMGMAREVSGRIVFMDQGIIVEMGSPAFFDNPSKLREPKQFISQPI

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

70.48

AAComp

91.25

DiffDock Confidence

-0.67

Seqdif (SeqID)

63.87 (WP_109459079)

Structdif (F-Target)

95.93 (4u00 A)

ProtNLM

Name	Score
Amino acid ABC transporter ATP-binding protein	0.303
Glutamine ABC transporter ATP-binding protein	0.199
Polar amino acid ABC transporter ATP-binding p...	0.060
Glutamine transport ATP-binding protein GlnQ	0.041
Amino acid ABC transporter ATP-binding protein...	0.034
Polar amino acid transport system ATP-binding ...	0.031
Amino acid ABC transporter ATPase	0.031
Amino acid ABC transporter ATP-binding protein...	0.030
ATP-binding cassette domain-containing protein	0.026
ABC transporter domain-containing protein	0.022

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

8_-0.81 | 18