M75CM

Prompt

The protein includes an ABC-transporter extension domain and is involved in the negative regulation of metabolic processes. These features suggest it helps transport molecules across membranes and slows down metabolic activities.

AI Generated Sequence

231 AAs | 25.6 kDa | 27.42 kDa (with tag)

MLEVRNLSKSFGERVLFENVSFTVPAGERIGLVGRNGAGKSTLLKILAGEEPPDSGEITLPKGVRIGYLPQEAPFDEDTVLDEVMAGDFARLRALEAERAAAEAEDEAMRRVAELHERFAEIDGYAAEARAQALLHGLGFKPEDQQQPVSDFSGGWQMRVALAQVLMSKPDILLLDEPTNHLDIDSIVWLEEVLRRFPGAMVIISHDRDFLDRVVTHIVEVENGEITRYPG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

74.42

AAComp

92.49

DiffDock Confidence

-0.66

Seqdif (SeqID)

53.42 (HTO84813)

Structdif (F-Target)

80.69 (2pcj B)

ProtNLM

Name	Score
ABC-F family ATP-binding cassette domain-conta...	0.336
ABC transporter ATP-binding protein	0.258
Glycosyl transferase family 2	0.126
ABC transporter	0.054
ATP-binding cassette domain-containing protein	0.031
ABC transporter, ATP-binding protein	0.022
ABC transporter domain-containing protein	0.021
ATP-binding cassette subfamily F protein 3	0.021
Glycosyl transferase family 1	0.015
ABC transporter ATP-binding protein YheS	0.013

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.16 (Lunatic)	8	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.08 (Lunatic)	4	100	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

67atp_-0.79t | 16