M4WKJ

Prompt

This protein has RNA helicase and ATP-binding activities, crucial for RNA processing and splicing.

AI Generated Sequence

372 AAs | 41.15 kDa | 42.97 kDa (with tag)

MTFSSLGLSAPLLRAVAEQGYTEPTPIQQQAIPAVLEGRDVMACAQTGTGKTAGFALPMLQRLAEGRARARRMPVRALVLAPTRELAAQVAESFRAYGKHLPLRVLLVYGGVSINPQMMKLRGGVDILVATPGRLLDHFEQRALKLSQVEFLVLDEADRMLDMGFIHDIRRIMALLPKRRQTLFFSATMSPEIRALADRFLHNPVRIEVAPRNSTAETVEQRVVPVDKARKRALLSHLLRERNPEQALVFSRTKHGANRLAKELEKDGIPAEAIHGNKSQGARTRALADFKAGDIRVLVATDVAARGLDIDELPHVFNFDLPNVAEDYVHRIGRTGRAGATGEAFSFVAADEAKLLRDIEKLIQKLIPRVEE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

72.25

AAComp

92.4

DiffDock Confidence

-1.02

Seqdif (SeqID)

72.24 (WP_160539079)

Structdif (F-Target)

87.02 (8enk A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.456
ATP-dependent RNA helicase RhlE	0.304
ATP-dependent helicase	0.067
3.6.4.13	0.049
RNA helicase	0.021
DEAD/DEAH box helicase domain protein	0.019
ATP-dependent RNA helicase	0.017
3.6.1.-	0.012
ATP-dependent RNA helicase rhlE	0.006
Superfamily II DNA/RNA helicase	0.004

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	77.2	76.9

Data

65_-1.27t | 97