MSPS6

Prompt

The protein features a ATP-dependent RNA helicase DEAD-box, conserved site and a cellular macromolecule catabolic process.

AI Generated Sequence

292 AAs | 31.7 kDa | 33.52 kDa (with tag)

MTTTPFADLPLSPALLAALDALGYEEPTPIQAQALPALLAGRDVIGQAKTGSGKTAAFGLPLLQRIDPARHAPQALVLCPTRELAIQVAREIRRLARYIPNIKVLTLCGGQPIGPQIRSLEHGAHIVVGTPGRILDHLERGTLKLDGLNTLVLDEADRMLDMGFQDAIDAICAQTPKDRQTLLFSATFPPAIAKIARRQMRDPVEITVAAEHADAPIEQRFYEVAPGERLHALGRLLDHYEPASALAFCNTKRDVDTLAATLQARGISALALHGELDQRERDQVLMQFANRS

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

72.45

AAComp

90.24

DiffDock Confidence

-1.01

Seqdif (SeqID)

68.38 (WP_115537694)

Structdif (F-Target)

67.17 (5gju A)

ProtNLM

Name	Score
ATP-dependent RNA helicase DbpA	0.596
DEAD/DEAH box helicase	0.210
3.6.4.13	0.088
ATP-dependent RNA helicase	0.033
ATP-independent RNA helicase DbpA	0.009
DEAD/DEAH box helicase domain protein	0.006
ATP-dependent 23S rRNA helicase DbpA	0.005
RNA helicase	0.003
3.6.1.-	0.003
Cold-shock DEAD box protein A	0.002

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	1 (Lunatic)	50	49.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.5 (Lunatic)	25	98.6	N/A	N/A
Tierra	T3	Biologics Optimized System	1.18 (Lunatic)	59	55.3	N/A	N/A
Tierra	T4	Biologics Optimized System	1.04 (Lunatic)	52	98.4	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

58atp_-1.19t | 89