MZ1F4

Prompt

The protein features a DNA2/NAM7 helicase-like, C-terminal and a RNA helicase activity.

AI Generated Sequence

199 AAs | 22.11 kDa | 23.93 kDa (with tag)

GEKGEKARLLAQIIRELEPEQVIVFTNTLHDVDALAKHLDDQGVPVTALHGDRSQAQREAMLKEFKSGTTRPVLVATPRELAVQVAEEVLKLGGAAGLRVAHVVYGGVNFPPQIRELKRGAHIVIATPGRLLDHLERAHFTVQHLKTLVLDEADEMLDMGFRPTIRKILGKLPKARQTVMFSATMPEEISQFAKDFMFD

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

76.22

AAComp

92.58

DiffDock Confidence

-1.36

Seqdif (SeqID)

55.3 (MCB1163639)

Structdif (F-Target)

56.78 (5gvs D)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.724
ATP-dependent RNA helicase	0.131
ATP-dependent helicase	0.075
RNA helicase	0.010
Helicase ATP-binding domain-containing protein	0.009
ATP-dependent RNA helicase RhlE	0.007
DEAD/DEAH box helicase domain protein	0.005
Helicase	0.004
DEAD/DEAH RNA helicase	0.004
DEAD-box ATP-dependent RNA helicase CshA	0.004

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	0.23 (Lunatic)	11.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	0.166 (A660nm)	21.58	>95	low confidence	low confidence

Data

61_-1.21t | 38