MGLVB

Prompt

The protein features a nucleoside phosphorylase domain involved in AMP salvage.

AI Generated Sequence

192 AAs | 20.21 kDa | 22.03 kDa (with tag)

MRILILGAPGAGKGTQAALLADKLGIPHISTGDLLAANVAAGTELGVKAKEIMAAGELVSDELIVGLMSDRIAQEDAAKGFILDGYPRTPQAAALAKKLPADGVVLDAAVRLDKVNEALVEGRMARAAEDGRADDNEAEAFAKRLKVYRTETAPVADFYRSDGKHLAVDGMRSVEEVTAEMEAYLARLAKLK

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

62.84

AAComp

87.66

DiffDock Confidence

-0.86

Seqdif (SeqID)

54.3 (MFK7898005)

Structdif (F-Target)

90.33 (5x6k B)

ProtNLM

Name	Score
AK	0.242
Adenylate kinase	0.189
2.7.4.3	0.149
Adenylate monophosphate kinase	0.147
ATP:AMP phosphotransferase	0.139
ATP-AMP transphosphorylase	0.130
ADK_lid domain-containing protein	0.000
Nucleoside monophosphate kinase	0.000
Polyphosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.6 (Lunatic)	30	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.1 (Lunatic)	5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.45 (Lunatic)	22.5	92	N/A	N/A
Tierra	T4	Biologics Optimized System	0.29 (Lunatic)	14.5	96.3	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

53atp_-1.18 | 47