MG4ML

Prompt

The protein contains a PRD domain and an RNA polymerase sigma factor 54 interaction domain. These domains facilitate specific protein functions and interactions.

AI Generated Sequence

248 AAs | 27.17 kDa | 28.99 kDa (with tag)

MSELERLREYLAATLADPLAATLAEVERAAAVDVSTIIGRSTGVGKELAARAHRDLSPRARGPFVAVNCAALPESLFESLFGYERAGSFTGAERQRIGLLEALAGGTLFLDEIGDMPLSLQAKLLRVLEERGVRRVGGRTPIEVDVRVIAATNRDLEAAVRAGRFREDLFYRLNVVPLHLPPLRERREDIPLLVDHFLAEFARRRGRPVPSLSAEALAALRAYPWPGNVRELENVIERAAVLAGGEVE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

67.83

AAComp

87.37

DiffDock Confidence

-0.67

Seqdif (SeqID)

57.76 (WP_013908400)

Structdif (F-Target)

90.41 (6luf D)

ProtNLM

Name	Score
Sigma-54-dependent Fis family transcriptional ...	0.314
Sigma-54 factor interaction domain-containing ...	0.314
Fis family transcriptional regulator	0.097
Transcriptional regulatory protein ZraR	0.033
Sigma54 specific transcriptional regulator, Fi...	0.018
DNA-binding response regulator	0.011
Two component, sigma54 specific, transcription...	0.010
Sigma 54-interacting transcriptional regulator	0.008
Response regulator	0.008
Response regulator of zinc sigma-54-dependent ...	0.007

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	low confidence	low confidence

Data

0atp_-1.16t | 88