M21ZT

Prompt

The protein features a Transport-associated OB, type 2 and a carbohydrate transport.

AI Generated Sequence

357 AAs | 38.1 kDa | 39.92 kDa (with tag)

MASVELRNVRKSFGEVEVIKGIDLEIADGEFVVFVGPSGCGKSTLLRMIAGLEDITGGEILIDGRRVNDVPPAKRGIAMVFQSYALYPHMTVAENIGFGLKLAGVPKAEIDARVAEAARILQLTPLLDRKPKQLSGGQRQRVAIGRAIVREPKAFLFDEPLSNLDAELRVQMRLEIAKLHRELGTTMIYVTHDQVEAMTMADRIVVLNAGRIEQVGSPLELYDRPANRFVAGFIGSPKMNFLPGTVEAAGGEVRVELAGGTRIALPAGAAAAAGEAVTLGIRPEHLRVAAEGGAALEGTVEVAEHLGSDTHLHLRLAGGELTARVEGEFALRPGDRVALAPDARAVHLFDAAGRALR

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

66.92

AAComp

91.16

DiffDock Confidence

-0.79

Seqdif (SeqID)

65.46 (WP_408596300)

Structdif (F-Target)

95.49 (7x0q A)

ProtNLM

Name	Score
Sn-glycerol-3-phosphate ABC transporter ATP-bi...	0.187
Sugar ABC transporter ATP-binding protein	0.148
ABC transporter ATP-binding protein	0.111
Multiple sugar transport system ATP-binding pr...	0.106
3.6.3.19	0.045
Maltose/maltodextrin import ATP-binding protei...	0.044
ABC transporter	0.034
Carbohydrate ABC transporter ATP-binding prote...	0.031
ABC transporter related	0.027
Carbohydrate ABC transporter ATP-binding prote...	0.027

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.52 (Lunatic)	26	97.2	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.71 (Lunatic)	35.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.52 (Lunatic)	26	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.56 (Lunatic)	28	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

44atp_-0.87 | 13