M1CE2

Prompt

The protein features a DNA2/NAM7 helicase-like, C-terminal and a RNA helicase activity.

AI Generated Sequence

199 AAs | 22.38 kDa | 24.2 kDa (with tag)

GEKGEKARLLAQIIRELEPEQVIVFTNTLHDVDALAKHLDDQGVPVTALHGDRSQYQREAMLKEFKSGTTRPVLVATPRELAVQVYEEVLKLGGAAGCRVCHVVYGGVNFPPQIRELKRGAHIVIATPGRLLDHLERAHFTVQHLKYLVLDEADEMLDMGFRPTIRKILGKLPKARQTVMFSATMPEEISQFAKDFMFD

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

71.07

AAComp

93.11

DiffDock Confidence

-0.51

Seqdif (SeqID)

56.06 (MCB1163639)

Structdif (F-Target)

58.21 (6aib A)

ProtNLM

Name	Score
DEAD/DEAH box helicase	0.562
ATP-dependent RNA helicase	0.140
ATP-dependent helicase	0.129
Helicase ATP-binding domain-containing protein	0.061
RNA helicase	0.019
DEAD-box ATP-dependent RNA helicase CshA	0.009
3.6.4.13	0.008
DEAD/DEAH box helicase domain protein	0.008
ATP-dependent RNA helicase RhlB	0.007
DEAD/DEAH box helicase domain-containing protein	0.006

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Enzyme Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T3	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Tierra	T4	Biologics Optimized System	N/A	N/A	N/A	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	62.4	70.1

Data

61atp_-0.84t | 25